General amino acid permease (GAP1) activity was evaluated in adenylate
cyclase-deficient Saccharomyces cerevisiae to determine the effect of
cAMP levels on GAP1 activity. Lowering cAMP concentrations in the cul
ture media led to a decrease in the initial rates of L-citrulline upta
ke. Kinetics of the amino acid transport system showed a partial loss
of transport capacity, with no apparent modifications in permease affi
nity.