BORRELIA-BURGDORFERI URIDINE KINASE - AN ENZYME OF THE PYRIMIDINE SALVAGE PATHWAY FOR ENDOGENOUS USE OF NUCLEOTIDES

Authors
BOURSAUXEUDE C MARGARITA D GILLES AM BARZU O SAINTGIRONS I
Citation
C. Boursauxeude et al., BORRELIA-BURGDORFERI URIDINE KINASE - AN ENZYME OF THE PYRIMIDINE SALVAGE PATHWAY FOR ENDOGENOUS USE OF NUCLEOTIDES, FEMS microbiology letters, 151(2), 1997, pp. 257-261
Citations number
21
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
151
Issue
2
Year of publication
1997
Pages
257 - 261
Database
ISI
SICI code
0378-1097(1997)151:2<257:BUK-AE>2.0.ZU;2-4
Abstract
The 621 bp udk gene encoding Borrelia burgdorferi potential uridine ki nase, involved in the pyrimidine salvage pathway, was cloned and seque nced. The B. burgdorferi protein has a molecular mass of 24 kDa in sod ium dodecyl sulfate-polyacrylamide gel. The N-terminal sequence of the protein, Ala-Lys-Ile-Ile, is identical to that predicted but lacks N- terminal methionine. udk is located at around 15 kb from the left telo mere and forms an operon with an upstream ORF. A likely hypothesis for the role of the pyrimidine salvage pathway is the sole use of endogen ous nucleotides for Borrelia.