A. Deshmukh et al., Development and characterization of monoclonal antibodies to chicken riboflavin carrier protein, HYBRIDOMA, 19(5), 2000, pp. 387-395
Several monoclonal antibodies (MAbs) specific to chicken riboflavin carrier
protein (cRCP) were developed and characterized. Of the several MAbs analy
zed, four were directed against nonoverlapping epitopes as demonstrated by
MAb inhibition assay. Many of these epitopes appeared to be in close proxim
ity and only three were situated at distinct part of the molecule as reveal
ed by sandwich assay. A combination of chemical modification, peptide cleav
age by chemical and enzymatic methods, was used to analyze the possible ant
igenic structure recognized by these MAbs. An assembled epitope spanning th
e region 22-87 forms the antigenic site recognized by 4999.1; while MAb 555
5.3 interacted with the C-terminal peptide 203-219.