Simultaneous determination of bergenin and gallic acid in Bergenia ligulata wall by high-performance thin-layer chromatography

A simple and reproducible high-performance thin-layer chromatographic metho d was developed for. the simultaneous determination of bergenin and gallic acid in Bergenia ligulata. Water and methanol were used as the extracting s olvents. The concentrations of bergenin and gallic acid in both of these so lvents were found to be almost the same. The method involves separation of the components by thin-layer chromatography on a precoated Silica Gel 60 F- 254 plate with a solvent system of ethyl acetate-formic acid-acetic acid-wa ter (100 + 11 + 11 + 27). The sensitivity of the method for bergenin was 0. 30 mug, whereas for gallic acid it was 0.25 mug. The proposed method is pre cise and sensitive and can be used for the detection, monitoring, and simul taneous quantification of bergenin and gallic acid in B. ligulata.