Expression of truncated transient receptor potential protein 1 alpha (Trp1alpha) - Evidence that the Trp1 C terminus modulates store-operated Ca2+ entry
Bb. Singh et al., Expression of truncated transient receptor potential protein 1 alpha (Trp1alpha) - Evidence that the Trp1 C terminus modulates store-operated Ca2+ entry, J BIOL CHEM, 275(47), 2000, pp. 36483-36486
Transient receptor potential protein 1 (Trp1) has been proposed as a compon
ent of the store-operated Ca2+ entry (SOCE) channel. However, the exact mec
hanism by which Trp1 is regulated by store depletion is not known. Here, we
examined the role of the Trpl C-terminal domain in SOCE by expressing hTrp
1 alpha lacking amino acids 664-793 (Delta Trp1 alpha) or full-length hTrp1
alpha in the HSG (human submandibular gland) cell line. Both carbachol (CC
h) and thapsigargin (Tg) activated sustained Ca2+ influx in control (nontra
nsfected), Delta Trp1 alpha-, and Trp1 alpha -expressing cells. Sustained [
Ca2+](i), following stimulation with either Tg or CCh in Delta Trp1 alpha -
expressing cells, was about 1.5-2-fold higher than in Trp1 alpha -expressin
g cells and 4-fold higher than in control cells. Importantly, (i) basal Ca2
+ influx and (ii) Tg- or CCh-stimulated internal Ca2+ release were similar
in all the cells. A similar increase in Tg-stimulated Ca2+ influx was seen
in cells expressing Delta 2Tr1 alpha, lacking the C-terminal domain amino a
cid 649-793, which includes the EWKFAR sequence. Further, both inositol 1,4
,5-trisphosphate receptor-3 and caveolin-1 were immunoprecipitated with Del
ta Trp1 alpha and Trp1 alpha. In aggregate, these data suggest that (i) the
EWKFAR sequence does not contribute significantly to the Trp1-associated i
ncrease in SOCE, and (ii) the Trpl C-terminal region, amino acids 664-793,
is involved in the modulation of SOCE.