Sa. Titus et Rg. Moran, Retrovirally mediated complementation of the glyB phenotype - Cloning of ahuman gene encoding the carrier for entry of folates into mitochondria, J BIOL CHEM, 275(47), 2000, pp. 36811-36817
The transduction of a human placental cDNA retroviral library into glyB cel
ls, a Chinese hamster ovary RI subline that is deficient in the transport o
f folates into mitochondria, resulted in the complementation of glycine aux
otrophy of these cells. A 2.6-kilobase pair cDNA insert flanked by retrovir
al sequences had integrated into genomic DNA in rescued cells. An open read
ing frame in this cDNA encoded a 35-kDa protein homologous to several inner
mitochondrial wall transporters for intermediate metabolites. The subclone
d cDNA complemented the glycine auxotrophy of glyB cells and reinstated fol
ate accumulation in the mitochondria of transfected cells, The human origin
, chromosomal location, and intron-exon organization of the isolated mitoch
ondrial folate transporter gene were deduced from the expressed sequence ta
g database and human genome project data.