Prostaglandin F-2 alpha-induced expression of 20 alpha-hydroxysteroid dehydrogenase involves the transcription factor NUR77

Prostaglandin F(2)alpha (PGF(2)alpha) binding to its receptor on the rat co rpus luteum triggers various signal transduction pathways that lead to the activation of a steroidogenic enzyme, 20 alpha -hydroxysteroid dehydrogenas e (20 alpha -HSD), which in turn catabolizes progesterone. The molecular me chanism underlying PGF(2)alpha -induced 20 alpha -HSD) enzyme activity has not yet been explored. In this report we show, using mice lacking PGF(2)alp ha receptor and pregnant rats, that PGF(2)alpha is responsible for the rapi d and massive expression of the 20 alpha -HSD gene at the end of pregnancy leading to a decrease in progesterone secretion. We also present evidence t hat PGF(2)alpha enhances 20 alpha -HSD promoter activity. We have determine d a region upstream of the -1590 position in the 20 alpha -HSD promoter tha t confers regulation by PGF(2)alpha in ovarian primary cells. This region e ncompasses a unique transcription factor-binding site with a sequence of a NUR77 response element. Deletion of this motif or overexpression of a NUR77 dominant negative protein caused a complete loss of 20 alpha -HSD promoter activation by PGF(2)alpha. NUR77 also transactivated the 20a-HSD promoter in transient transfection experiments in corpus luteum-derived cells (GG-CL ), This induction required the NUR77-transactivating domain. We also show t hat PGF(2)alpha induces a very rapid expression of NUR77 that binds to a di stal response element located at -1599/ -1606 but does not interact with an other proximal putative NUR77 response element located downstream in the pr omoter. A rapid increase in NUR77 mRNA was observed in mice corpora lutea j ust before parturition at a time when 20 alpha -HSD becomes expressed. This increase in the expression of both genes was not seen in PGF(2)alpha recep tor knockout mice. By using cyclosporin A and PGF(2)alpha treatment, we est ablished that inhibition of NUR77 DNA binding in vivo prevents PGF(2)alpha induction of the 20 alpha -HSD gene in the corpus luteum. Taken together, o ur results demonstrate, for the first time, that PGF(2)alpha induces in the corpus luteum the expression of the nuclear orphan receptor and transcript ion factor, NUR77, which in turn leads to the transcriptional stimulation o f 20 alpha -HSD, triggering the decrease in serum progesterone essential fo r parturition.