Cloning and sequencing of the deacetylase gene from Vibrio alginolyticus H-8

Citation
K. Ohishi et al., Cloning and sequencing of the deacetylase gene from Vibrio alginolyticus H-8, J BIOSCI BI, 90(5), 2000, pp. 561-563
Citations number
15
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
90
Issue
5
Year of publication
2000
Pages
561 - 563
Database
ISI
SICI code
1389-1723(200011)90:5<561:CASOTD>2.0.ZU;2-P
Abstract
A gene encoding deacetylase DA1 that is specific for N, N'-diacetylchitobio se was cloned using the shot-gun method with pUC118 and sequenced. The open reading frame encoded a protein of 427 amino acids including the signal pe ptide. The molecular mass of the mature enzyme estimated from the amino aci d sequence data was 44.7 kDa, which is approximately similar to that, estim ated by SDS-PAGE (48.0 kDa), of the purified enzyme reported previously. Th e N-terminal amino acid sequence deduced from the cloned deacetylase gene s howed partial sequence homology with the Nod B protein from Rhizobium sp. ( 37% identity) and chitin deacetylase from Mucor rouxii (28%). It contained a domain, which showed homology with a chitin-binding domain of chitinase A from Bacillus circulans (39%).