Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR

Authors
Honda, M Minetoki, T Gomi, K Machida, M
Citation
M. Honda et al., Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR, J BIOSCI BI, 90(5), 2000, pp. 577-579
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
90
Issue
5
Year of publication
2000
Pages
577 - 579
Database
ISI
SICI code
1389-1723(200011)90:5<577:RDOHID>2.0.ZU;2-S
Abstract
A simple and rapid method for the analysis of artificially introduced DNA f ragments has been developed using competitive PCR and long and accurate PCR . The locus and the copy number of the DNA fragments in each Aspergillus or yzae transformant could be detected more rapidly and accurately by this met hod than by the conventional Southern hybridization method.