Validation of a high-performance liquid chromatographic method for the determination of ibuprofen enantiomers in plasma of broiler chickens

To characterise the pharmacokinetic properties of each enantiomer of ibupro fen in broiler chickens, a stereospecific HPLC method based on a alpha (1)- acid glycoprotein bonded chiral stationary phase has been validated. S-(+)- naproxen was used as internal standard. Enantiomers of ibuprofen and S-(+)- naproxen were baseline separated using a mobile phase consisting of 0.1 M p hosphate buffer pH=7 and 0.4% 2-propanol. The method is precise, specific, accurate and reproducible. Recoveries were higher than 80% and the limits o f quantification for R-(-)- and S-(+)-ibuprofen were 1.16 and 1.37 mug ml(- 1), respectively. The method seemed suitable fur the pharmacokinetic studie s of ibuprofen in chickens. (C) 2000 Elsevier Science B.V. All rights reser ved.