Penetration pathways of fluorescent dyes in human hair fibres investigatedby scanning near-field optical microscopy

Citation
A. Kelch et al., Penetration pathways of fluorescent dyes in human hair fibres investigatedby scanning near-field optical microscopy, J MICROSC O, 200, 2000, pp. 179-186
Citations number
19
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF MICROSCOPY-OXFORD
ISSN journal
00222720 → ACNP
Volume
200
Year of publication
2000
Part
3
Pages
179 - 186
Database
ISI
SICI code
0022-2720(200012)200:<179:PPOFDI>2.0.ZU;2-D
Abstract
Thin cross-sections of human hairs were investigated by scanning near-field optical microscopy (SNOM) and confocal laser scanning microscopy (CLSM) af ter penetration of a fluorescent dye. The same samples were measured with b oth techniques to compare the observed structures. The images obtained from the two methods show nearly identical structures representing pathways of the dye molecules in hairs. The SNOM images provide a higher resolution tha n the CLSM images. Therefore, SNOM is believed to be a suitable method for investigations at a resolution of 100 nm on penetration pathways of fluores cent dyes such as the cell membrane complex pathway in cross-sections of ha irs.