Adaptive aberration correction in a two-photon microscope

Authors
Neil, MAA Juskaitis, R Booth, MJ Wilson, T Tanaka, T Kawata, S
Citation
Maa. Neil et al., Adaptive aberration correction in a two-photon microscope, J MICROSC O, 200, 2000, pp. 105-108
Citations number
19
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF MICROSCOPY-OXFORD
ISSN journal
00222720 → ACNP
Volume
200
Year of publication
2000
Part
2
Pages
105 - 108
Database
ISI
SICI code
0022-2720(200011)200:<105:AACIAT>2.0.ZU;2-0
Abstract
We demonstrate aberration correction in two-photon microscopy. Specimen-ind uced aberrations were measured with a modal wavefront sensor, implemented u sing a ferroelectric liquid crystal spatial light modulator (FLCSLM). Wavef ront correction was performed using the same FLCSLM. Axial scanned (x-z) im ages of fluorescently labelled polystyrene beads using an oil immersion len s show restored sectioning ability at a depth of 28 mum in an aqueous speci men.