Three-dimensional force calibration of optical tweezers

Authors
Singer, W Bernet, S Hecker, N Ritsch-Marte, M
Citation
W. Singer et al., Three-dimensional force calibration of optical tweezers, J MOD OPT, 47(14-15), 2000, pp. 2921-2931
Citations number
11
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science","Optics & Acoustics
Journal title
JOURNAL OF MODERN OPTICS
ISSN journal
09500340 → ACNP
Volume
47
Issue
14-15
Year of publication
2000
Pages
2921 - 2931
Database
ISI
SICI code
0950-0340(200011)47:14-15<2921:TFCOOT>2.0.ZU;2-D
Abstract
We demonstrate a method for 3-dimensional force calibration of optical twee zers by recording the trapping dynamics of polystyrene beads. This is reali zed by time-resolved detection of the horizontal and vertical position of a bead which is drawn to the focus of a laser beam. The method provides real time characterization of the force profile of an optical trap in all direc tions.