ANALYSIS OF NUCLEOTIDE POOLS IN HUMAN LYMPHOMA-CELLS BY CAPILLARY ELECTROPHORESIS

Capillary electrophoresis is applied to determine nucleotide pool leve ls in human Burkitt lymphoma cells. The analysis was performed on a 65 cm x 50 mu m I.D. Ucon-coated column with on-column UV detector. The method requires only nanoliters of sample and a simple sample preparat ion procedure. Over 12 nucleotides were separated and quantitated with high resolution and reproducibility. The whole capillary electrophore tic separation time was only 35 min, These results demonstrate that ca pillary electrophoresis provides a useful and easy way to analyze nucl eotide pools in cells.