Localization of Na-23(+) in a DNA quadruplex by high-field solid-state NMR

Citation
D. Rovnyak et al., Localization of Na-23(+) in a DNA quadruplex by high-field solid-state NMR, J AM CHEM S, 122(46), 2000, pp. 11423-11429
Citations number
53
Categorie Soggetti
Chemistry & Analysis",Chemistry
Journal title
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN journal
00027863 → ACNP
Volume
122
Issue
46
Year of publication
2000
Pages
11423 - 11429
Database
ISI
SICI code
0002-7863(20001122)122:46<11423:LONIAD>2.0.ZU;2-O
Abstract
Monovalent cations such as Na+, K+, and NH4+ are known to stabilize DNA qua druplexes formed of guanine-quartets. Such structures readily form from the guanine-rich repeat sequences found in telomeres, the physical ends of euk aryotic chromosomes. We present a solid-state NMR approach for studying ion s associated with G-quartets based on the direct NMR observation of Na-23() ion resonances and report new methods for studying quadrupolar nuclei in biological solids. In the tetraplex forming oligonucleotide d(TG(4)T), high -field (17.6 T) NMR spectra cleanly resolve three distinct classes of sodiu m ions. A high-resolution 2D-MQMAS spectrum established the assignment of a n amorphously broadened signal at -19 ppm (relative to 0.1 M NaCl) to surfa ce-bound Na+ ions. Two-dimensional nutation spectroscopy was used to indica te the relative size of the quadrupole coupling for each line, while a stan dard exchange experiment established a correlation between surface (-19 ppm ) and channel-bound (6.8 ppm) Na+ ions. Finally, free sodium ions are obser ved at 0 ppm. This work demonstrates the utility of high field spectroscopy in combination with a suite of 2D solid-state NMR experiments for resolvin g and assigning multiple Na+ sites in [d(TG(4)T)](4).