Time-course magnetic resonance imaging of rat pancreatic cyst after experimental pancreatitis

Fast magnetic resonance (MR) imaging of the rat pancreas was carried out us ing a snapshot method to observe three-dimensional (3D) and temporal develo pment of the pancreatic cyst after experimental pancreatitis. Acute pancrea titis was induced by a retrograde infusion of the trypsin-taurocholate solu tion into the pancreatic duct in 23 rats, of which seven survived for one m onth. Under 2% enflurane anesthesia, H-1 images of the rat abdomen were tak en by a 4.7 T magnetic resonance spectrometer under spontaneous breathing. 3D images of the pancreas and cyst were reconstructed from the axial, sagit tal and coronal images taken before, 24 h, 7 days, 14 days, 21 days and 28 days after the induction of pancreatitis. The 3D images reconstructed from different slice orientations at each rime point showed good agreement with each other. The calculated volumes of the cyst on 7th, 14th, 21st, and 28th day were 0.3 +/- 0.1, 0.8 +/- 0.3, 2.1 +/- 0.6, 6.5 +/- 1.3 mL, respective ly. The cystic fluid volume on 28th day was 6.4 +/- 1.4 mL, which confirmed reliability of volume measurement by MR imaging. Fast MR imaging (snapshot ) together with 3D reconstruction allows us to understand the detailed chro nological and spatial development of pancreatic cyst after acute pancreatit is in rats. (C) 2000 Elsevier Science Inc. All rights reserved.