Optimal fluorescence excitation wavelengths for detection of squamous intra-epithelial neoplasia: results from an animal model

Using the hamster cheek pouch carcinogenesis model, we explore which fluore scence excitation wavelengths are useful for the detection of neoplasia. 42 hamsters were treated with DMBA to induce carcinogenesis, and 20 control a nimals were treated only with mineral oil. Fluorescence excitation emission matrices were measured from the cheek pouches of the hamsters weekly. Resu lts showed increased fluorescence near 350-370 nm and 410 nm excitation and decreased fluorescence near 450-470 nm excitation with neoplasia. The opti mal diagnostic excitation wavelengths identified using this model - 350-370 nm excitation and 400-450 nm excitation - are similar to those identified for detection of human oral cavity neoplasia. (C) 2000 Optical Society of A merica.