Synthesis and biophysical characterization of tRNA(LyS,3) anticodon stem-loop RNAs containing the mcm(5)s(2)U nucleoside

Authors
Bajji, AC Davis, DR
Citation
Ac. Bajji et Dr. Davis, Synthesis and biophysical characterization of tRNA(LyS,3) anticodon stem-loop RNAs containing the mcm(5)s(2)U nucleoside, ORG LETT, 2(24), 2000, pp. 3865-3868
Citations number
34
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
ORGANIC LETTERS
ISSN journal
15237060 → ACNP
Volume
2
Issue
24
Year of publication
2000
Pages
3865 - 3868
Database
ISI
SICI code
1523-7060(20001130)2:24<3865:SABCOT>2.0.ZU;2-3
Abstract
Phosphoramidite reagents of the naturally occurring modified nucleosides mc m(5)s(2)U and mcm(5)U were synthesized and along with pseudouridine were in corporated into 17-nucleotide lysine tRNA anticodon stem-loop domains. Stan dard RNA phosphoramidite coupling chemistry allowed us to systematically in vestigate the thermodynamic effects of nucleoside modification and to corre late thermodynamic trends with qualitative structure effects seen by NMR sp ectroscopy.