The use of PeptiCLEC-TR in the preparation of dipeptides

Dipeptides are important intermediates in many pharmaceutical products. To support a research programme on matrix metalloproteinases (MMP) we needed t o prepare the dipeptide, Leu-Phe-NHMe (3). Chemical methods provided the ma terial but not to the quality required, and thus an alternative preparation was sought. The use of enzymes in chemical synthesis was a tool that we ha d used before but not in the preparation of dipeptides, This contribution w ill show the utility of PeptiCLEC-TR in the preparation of dipeptide (3) an d its application to other dipeptides, It will highlight the amenability of PeptiCLEC-TR to scale-up and demonstrate the recycling of this enzyme, whi ch will make this a cost-effective route.