Effects of intrinsic denervation on intestinal morphology in rats with short-bowel syndrome

When benzalkonium chloride solution (BACs) is locally applied, to the seros al surface of the intestine, it causes intrinsic denervation (ID) of the my enteric plexus (MP), changes intestinal morphology, and slows intestinal pa ssage by prolonging small-bowel transit time. These effects of ID suggest t hat chemically-induced bowel denervation may be useful in the treatment of short-bowel syndrome (SBS). How ID affects intestinal morphology in rats wi th SBS has not previously been investigated. A 75%-80% mid-small-bowel rese ction was performed in 20 rats with mean body weight 247 +/- 30 g. The prox imal and distal 2 cm of the resected bowel were examined histologically. Af ter intestinal continuity was maintained by end-to-end anastomosis, a 2-cm jejunal segment was marked with silk sutures to form the test segment. BACs 0.1% was applied to 10 of the 20 test segments according to the modified F ox method, resulting in MP destruction (G1). Saline solution was applied to the other 10 test segments to form the control group (G2). Three months la ter, the rats were killed and the jejunal, ileal, and test segments were ev aluated morphologically. Results were expressed as mean +/- standard deviat ion. The Wilcoxon parametric test was performed to compare the groups durin g the operation and after death, while the Mann-Whitney U-test was used to compare the data in GI and G2. No intestinal obstruction was observed in ei ther group. In G1, the body weight increased by 19.1% and the total small-i ntestinal lengthening was 62.2% (P < 0.05). In the test segment of G1, 75% of the ganglia in the MP were destroyed and villus height, crypt depth, int estinal muscle thickness, number of enterocytes, and villus density increas ed compared to G2. In the ileal segments of G1, there was an increase of 28 .8% in intestinal diameter, 14% in muscle thickness, and 15% in villus dens ity (P < 0.05). No change was observed in the untreated jejunal segments of G1 and G2. Thus, ID of the MP after segmental BACs application of the jeju nal level: (1) does not cause intestinal obstruction after 3 months; (2) th e increase in bowel diameter in the test and ileal segments increases the a bsorptive surface of the mucosa; (3) the morphologic changes in the test an d ileal segments verify an increase in intestinal adaptation; and (4) BACs application in rats with SBS is an easy procedure with no morbidity or mort ality, and can be used to increase intestinal adaptation in rats with SBS.