Nonradioactive mRNA differential display in polyacrylamide mini-gels

Nonradioactive methods in comparison to those using radionuclides are now b ecoming popular because of safety, environmental, and cost concerns. The mo dified technique described here is much faster, easier, and safer than the conventional procedure utilizing radioactive material and large-formatted p olyacrylamide gel, or other nonradioactive DD techniques. Also, it maintain s good reproducibility and specificity. This DD protocol offers a rapid and reliable nonradioactive method for comparing gene expression patterns in v arious research.