Nonradioactive methods in comparison to those using radionuclides are now b
ecoming popular because of safety, environmental, and cost concerns. The mo
dified technique described here is much faster, easier, and safer than the
conventional procedure utilizing radioactive material and large-formatted p
olyacrylamide gel, or other nonradioactive DD techniques. Also, it maintain
s good reproducibility and specificity. This DD protocol offers a rapid and
reliable nonradioactive method for comparing gene expression patterns in v
arious research.