Quantitative measurement of muscle hemoglobin oxygenation using near-infrared spectroscopy with correction for the influence of a subcutaneous fat layer
M. Niwayama et al., Quantitative measurement of muscle hemoglobin oxygenation using near-infrared spectroscopy with correction for the influence of a subcutaneous fat layer, REV SCI INS, 71(12), 2000, pp. 4571-4575
The inhomogeneity of tissue structure greatly affects the sensitivity of ti
ssue oxygenation measurement by reflectance near-infrared spectroscopy. In
this study, we investigated the influence of a fat layer on muscle oxygenat
ion measurement by in vivo tests and Monte Carlo simulation, and we propose
a method for correcting the influence. In the simulation, a three-dimensio
nal model consisting of the epidermis, dermis, fat, and muscle layers was u
sed. In in vivo tests, measurement sensitivity was examined by measuring ox
ygen consumption of the forearm muscle and the peak-to-peak variation of ox
ygenation in periodic exercise tests on the vastus lateralis using a newly
developed multisensor type of tissue oximeter. Fat layer thickness was also
measured by ultrasonography. The correction curve of measurement sensitivi
ty against fat layer thickness was obtained from the results of simulation
and in vivo tests. The values of corrected oxygen consumption were almost t
he same and had less variation between individuals (0.13 +/-0.02 ml 100 g(-
1) min(-1)) than did the uncorrected values (0.08 +/-0.04 ml 100 g(-1) min(
-1)). (C) 2000 American Institute of Physics. [S0034-6748(00)02812-4].