HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF ONDANSETRON ENANTIOMERS IN HUMAN SERUM USING A REVERSED-PHASE CELLULOSE-BASED CHIRAL STATIONARY-PHASE AND SOLID-PHASE EXTRACTION
Jl. Liu et Jt. Stewart, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF ONDANSETRON ENANTIOMERS IN HUMAN SERUM USING A REVERSED-PHASE CELLULOSE-BASED CHIRAL STATIONARY-PHASE AND SOLID-PHASE EXTRACTION, Journal of chromatography B. Biomedical sciences and applications, 694(1), 1997, pp. 179-184
Citations number
10
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A stereospecific HPLC method was developed for the assay of R-(-)- and
S-(+)-ondansetron enantiomers in human serum. The method involves the
use of solid-phase extraction for sample clean-up and is also free of
interference from 6-hydroxyondansetron, 7-hydroxyondansetron and 8-hy
droxyondansetron, the three major metabolites of ondansetron. Chromato
graphic resolution of the enantiomers was performed on a reversed-phas
e cellulose-based chiral column (Chiralcel OD-R) under isocratic condi
tions using a mobile phase consisting of 0.7 M sodium perchlorate-acet
onitrile (65:35, v/v) at a how-rate of 0.5 ml/min. Recoveries at 200 n
g/ml levels were more than 90% for both ondansetron enantiomers. Intra
-day and inter-day precisions calculated as R.S.D.s were in the 0.3-5%
and 2-8% ranges for both enantiomers, respectively. Intra-day and int
er-day accuracies calculated as percent error were in the 0.3-11.5% an
d 0-3% ranges for both enantiomers, respectively. Linear calibration c
urves were obtained for each enantiomer in serum in the concentration
range 15-750 ng/ml. The limit of quantitation of each enantiomer was 1
5 ng/ml. The detection limit for each enantiomer in serum using UV det
ection at 210 nm was 7 ng/ml (S/N=2).