Vocal activity was studied in a Colombian highland-frog community, consisti
ng of five frog species belonging to three families with three different re
productive modes, which reproduced simultaneously in the area. We encounter
ed one diurnal and four nocturnal species. Each species had a distinct call
structure, and the 24-hour patterns of calling activity differed significa
ntly between all but two species. Interspecific differences in dominant cal
l frequencies corresponded inversely to differences in male snout-vent leng
th. Only one species called in ponds, whereas all other species used terres
trial sites. Among the terrestrial callers there seemed to be interspecific
differences in plant use as calling sites. Our results indicate that despi
te the low number of species, interspecific vocal partitioning is pronounce
d, and probably important for reliable vocal communication.