A mushroom (Agaricus bisporus) tissue homogenate based alcohol oxidase electrode for alcohol determination in serum

To construct homogenized tissue based biosensors by using plant tissue mate rials is a relatively new development in the biosensor technology. In this study, a homogenized mushroom (Agaricus bisporus) tissue based electrode in which alcohol oxidase activity was developed by immobilizing with gelatin and cross-linking agent glutaraldehyde at dissolved oxygen probe for determ ination of ethyl alcohol. The electrode response depends linearly on ethyl alcohol concentration between 0.2 and 20 mM. In the optimization studies, p hosphate buffer (pH 7.5. 50 mM) and 35 degreesC were obtained as the optimu m conditions. By using the electrode prepared we have made approximately 60 measurements in 10-h period, and response time of the electrode is 2 min f or each measurement. (C) 2000 Elsevier Science B.V. All rights reserved.