E. Akyilmaz et E. Dinckaya, A mushroom (Agaricus bisporus) tissue homogenate based alcohol oxidase electrode for alcohol determination in serum, TALANTA, 53(3), 2000, pp. 505-509
To construct homogenized tissue based biosensors by using plant tissue mate
rials is a relatively new development in the biosensor technology. In this
study, a homogenized mushroom (Agaricus bisporus) tissue based electrode in
which alcohol oxidase activity was developed by immobilizing with gelatin
and cross-linking agent glutaraldehyde at dissolved oxygen probe for determ
ination of ethyl alcohol. The electrode response depends linearly on ethyl
alcohol concentration between 0.2 and 20 mM. In the optimization studies, p
hosphate buffer (pH 7.5. 50 mM) and 35 degreesC were obtained as the optimu
m conditions. By using the electrode prepared we have made approximately 60
measurements in 10-h period, and response time of the electrode is 2 min f
or each measurement. (C) 2000 Elsevier Science B.V. All rights reserved.