Monitoring of mutagenic process with piezoelectric quartz crystal impedance analysis

Citation
Jz. Zhang et al., Monitoring of mutagenic process with piezoelectric quartz crystal impedance analysis, TALANTA, 53(3), 2000, pp. 525-533
Citations number
34
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
TALANTA
ISSN journal
00399140 → ACNP
Volume
53
Issue
3
Year of publication
2000
Pages
525 - 533
Database
ISI
SICI code
0039-9140(200012)53:3<525:MOMPWP>2.0.ZU;2-L
Abstract
A novel method for monitoring of mutagenic process of dimethyl sulfate to S almonella typhimurium strain (TA100) was proposed by using piezoelectric qu artz crystal impedance (PQCI) analysis technique. The time courses of respo nses piezoelectric impedance parameters for a quartz crystal in a culture s ystem were simultaneously obtained and discussed. It was found that the mot ional resistance variation (DeltaR(m)) increases and frequency shift (Delta f) of PQC sensor decreases correspondingly during the mutagenic process of the bacteria. These parameters could reflect the variations of viscosity an d density of culture system. By fitting DeltaR(m) versus time curves toward Gompertz bacterial growth model, we obtained and discussed the bacterial g rowth parameters for both normal growth and mutagenic process. The experime nts showed that the proposed method could provide real time and multidimens ional impedance information to the monitoring of mutagenic process. (C) 200 0 Elsevier Science B.V. All rights reserved.