T. Inami et al., Full-length nucleotide sequence of a simian TT virus isolate obtained froma chimpanzee: Evidence for a new TT virus-like species, VIROLOGY, 277(2), 2000, pp. 330-335
Recently, we identified TT virus (TTV) isolates from nonhuman primates and
named them simian TN (s-TTV). To characterize the genomic structure of thes
e isolates in more detail, the full-length nucleotide sequence of the s=TTV
isolate (designated s-TTV CH65-1), recovered from a chimpanzee born in Wes
t Africa, was amplified by nested PCR with inverted primers deduced from th
e untranslated region of s-TTV DNA. CH65-1 was composed of 3899 nucleotides
(nt) and had two open reading frames (ORF) spanning 2295 nt (ORF1) and 402
nt (ORF2). The sequence had only 52.3% similarity to the prototype TA278 h
uman isolate. Phylogenetic analysis demonstrated that CH65-1 was distinct f
rom the human TN isolates. These results suggested that s-TTV may represent
a new TTV-like viral species or genus. (C) 2000 Academic Press.