Ja. Burns et Hy. Wetzstein, DEVELOPMENT AND CHARACTERIZATION OF EMBRYOGENIC SUSPENSION-CULTURES OF PECAN, Plant cell, tissue and organ culture, 48(2), 1997, pp. 93-102
A method for the establishment and proliferation of developmentally st
able, embryogenic suspension cultures in pecan is described, and the g
rowth and development of cultures characterized. Suspension cultures w
ere generated from somatic embryos derived from zygotic embryo cotyled
on explants induced on a solidified medium with naphthaleneacetic acid
. Cultures were repetitively embryogenic and proliferated in growth-re
gulator-free medium. The suspensions consisted of a mixture of globula
r stage embryo-aggregates, freely suspended globular embryos and pre-g
lobular stage embryo masses. Culture growth and proembryo production w
ere evaluated with respect to several liquid media and pH conditions.
Significant differences in growth and productivity were observed betwe
en cultures. Pre-globular stage embryo masses collected on filter pape
r and overlaid on solidified medium continued ontological development
and converted into plants. Thus a method has been developed for pecan
suspension culture, which presents a major improvement in embryogenic
tissue culture within the Juglandaceae.