Gi. Hemre et T. Storebakken, Tissue and organ distribution of C-14-activity in dextrin-adapted Atlanticsalmon after oral administration of radiolabelled C-14(1)-glucose, AQUAC NUTR, 6(4), 2000, pp. 229-234
Accumulation of C-14 in various tissues and organs was studied in three dif
ferent groups of 0.8-kg Atlantic salmon Salmo salar force-fed with C-14(1)-
glucose in order to evaluate if metabolism of glucose depended on adaptatio
n to dietary carbohydrate level. The salmon had been fed diets supplemented
with 0, 100 and 200 g maize dextrin kg(-1) for 10 months before the experi
ment. The fish were force-fed 6.65 x 10(4) Bq of C-14(1) glucose kg(-1) BW,
in gelatin capsules. Fish for analysis were obtained 16 h later. C-14 was
measured in blood plasma, gill, kidney, liver and white muscle, and in lipi
d extract of liver.
The liver contained most C-14, followed by heart, blood plasma, gill and li
ver lipid extract, while kidney and muscle contained the least C-14 per gra
m or millilitre tissue. The muscle contained most radioactivity, on an esti
mated total tissue basis, followed by liver, blood plasma, gill, liver lipi
d extract, kidney and heart tissue. Thirty-eight per cent of the orally adm
inistered C-14 was recovered in the salmon adapted to the diet without dext
rin after 16 h. This was significantly (P < 0.05) higher than the 30% and 3
2% recovered in the salmon adapted to diets with 10% and 20% dextrin. This
effect on adaptation to dietary dextrin level in glucose uptake or metaboli
sm was supported by a trend (P < 0.10) toward higher radioactivity per gram
or millilitre of each individual tissue in the fish adapted to the diet wi
thout dextrin, when compared with the other two adaptation regimes.