H. Xiang et al., hTERT can function with rabbit telomerase RNA: Regulation of gene expression and attenuation of apoptosis, BIOC BIOP R, 278(3), 2000, pp. 503-510
Citations number
50
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Telomerase is a specialized DNA polymerase that adds telomeric sequences on
to chromosome ends. The functional telomerase complex contains a telomerase
reverse transcriptase (TERT) and also a telomerase RNA (TR). Although it i
s well established that the human telomerase reverse transcriptase (hTERT)
can function well in different human cell lines, it has not been shown whet
her it is compatible with telomerase template RNA from other species. Here
we report that the expressed hTERT is functionally compatible with rabbit t
elomerase template RNA (rTR) as demonstrated by TRAP assay. The direct inte
raction between hTERT and rTR is further confirmed by immunoprecipitation-l
inked RT-PCR in which rTR is detected from the complex immunoprecipitated b
y an antihTERT antibody. The hTERT expressed in rabbit lens epithelial cell
s demonstrates two major functions: modulation of expression of other genes
and attenuation of apoptosis. Thus, telomerase has a variety of functions
besides telomere synthesis, and the template RNA is functionally conserved
between human and rabbit. (C) 2000 Academic Press.