Segregation and linkage relationship of nine allozyme loci, which are fixed
for alternative alleles in the European fire-bellied toads, Bombina bombin
a and B. variegata, were studied using artificial F-1 hybrids to obtain bac
kcross and F-2 progeny. Alleles coding for electromorphs at nine loci (Ldh-
1, Mdh-1, Idh-1, Ck, Ak, Gpi, Aat-1, Np, and G6pd) showed Mendelian ratios.
Two of the loci, Ak and G6pd, were found to be closely linked (2 cM apart)
; the other loci assorted independently.