A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium

Carbohydrate-containing molecules were located on the surface of Candida li polytica by using ruthenium red in a cytochemical study. The yeast was grow n in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and begin ning of stationary growth phase. Control cultures were grown in a medium co ntaining glucose. The growth of the yeast in media containing glucose and T ween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pat tern also changed according to Tween 80 concentration. The influence of Twe en 80 on cellular carbohydrate expression is discussed.