Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene, GUT2, from Saccharomyces cerevisiae

Authors
Grauslund, M Ronnow, B
Citation
M. Grauslund et B. Ronnow, Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene, GUT2, from Saccharomyces cerevisiae, CAN J MICRO, 46(12), 2000, pp. 1096-1100
Citations number
29
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
CANADIAN JOURNAL OF MICROBIOLOGY
ISSN journal
00084166 → ACNP
Volume
46
Issue
12
Year of publication
2000
Pages
1096 - 1100
Database
ISI
SICI code
0008-4166(200012)46:12<1096:CSTROT>2.0.ZU;2-M
Abstract
Cytosolic glycerol kinase (Gut1p) and mitochondrial glycerol-3-phosphate de hydrogenase (Gut2p) constitute the glycerol utilization pathway in Saccharo myces cerevisiae. Transcriptional analysis of the GUT2 gene showed that it was repressed by glucose and derepressed on the non-fermentable carbon sour ces, glycerol, lactate and ethanol. Derepression of GUT2 requires the prote in kinase Snf1p as well as the heteromeric protein complex, Hap2/3/4/5, and its putative DNA-binding site (UAS(HAP)) located in the promoter region. F urthermore, glucose repression of GUT2 requires the negative regulator, Opi 1p.