Synthesis of alpha-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme

Authors
Fang, JW Chen, X Zhang, W Janczuk, A Wang, PG
Citation
Jw. Fang et al., Synthesis of alpha-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme, CARBOHY RES, 329(4), 2000, pp. 873-878
Citations number
20
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
329
Issue
4
Year of publication
2000
Pages
873 - 878
Database
ISI
SICI code
0008-6215(200012)329:4<873:SOAEDW>2.0.ZU;2-1
Abstract
beta -Gal epitopes are carbohydrate structures bearing an alpha -D-Galp-(1 --> 3)-beta -D-Galp terminus and are the main cause of antibody-mediated hy peracute rejection in xenotransplantation. Nine monosaccharides and ten dis accharides were evaluated as substrates for a fusion protein, which contain s both alpha-(1 --> 3)-galactosyltransferase and uridine-5'-diphosphogalact ose 4-epimerase. Four disaccharide and six trisaccharide alpha -Gal epitope derivatives were synthesized utilizing this novel fusion enzyme. (C) 2000 Elsevier Science Ltd. All rights reserved.