LC-MS analysis of the formed peptides from N-(O,O-diiso-propyl)phosphoryl aspartic acid

LC-ESI-MS method was used to analyze the formed di- and tri- peptide in the reaction system of N-(O,O-diisapropyl) phosphoryl aspartic add and adenosi ne. Cluster ions of the peptides were given in the ESI-MS. The structures o f these small peptides were confirmed by LC-MS-MS analysis. Compared with t he traditional HPLC-UV detection, this method showed good sensitivity and s electivity for peptide in the presence of compounds with strong UV absorpti on, such as nucleoside aml nucleotide.