SEPARATION AND ANALYSIS OF CYCLODEXTRINS BY CAPILLARY ELECTROPHORESISWITH DYNAMIC FLUORESCENCE LABELING AND DETECTION

Mixtures of alpha-, beta- and gamma-cyclodextrins were complexed with 2-anilinonaphthalene-6-sulfonic acid (2,6-ANS) and separated by capill ary electrophoresis. The enhanced fluorescence emission of 2,6-ANS whe n complexed with the cyclodextrins allowed isolated zones to be visual ized. Detection limits for alpha-, beta- and gamma-cyclodextrin were d etermined to be 62, 2.4, and 24 mu M, respectively. Enhanced resolutio n of cyclodextrin mixtures could be obtained by adjusting the concentr ation of ANS in the running buffer or by suppressing the electroosmoti c flow. Components in 2,6-di-O-methyl-beta-cyclodextrin with differing degrees of substitution were separated by this technique and compared with electrospray mass spectra of the same mixture.