O. Muraoka et al., Kheper, a novel ZFH/delta EF1 family member, regulates the development of the neuroectoderm of zebrafish (Danio rerio), DEVELOP BIO, 228(1), 2000, pp. 29-40
Kheper is a novel member of the ZFH (zinc-finger and homeodomain protein)/d
elta EF1 family in zebrafish. kheper transcripts are first detected in the
epiblast of the dorsal blastoderm margin at the early gastrula stage and kh
eper is expressed in nearly all the neuroectoderm at later stages. kheper e
xpression was expanded in noggin RNA-injected embryos and also in swirl mut
ant embryos and was reduced in bmp4 RNA-injected embryos and chordino mutan
t embryos, suggesting that kheper acts downstream of the neural inducers No
ggin and Chordino. Overexpression of Kheper elicited ectopic expansion of t
he neuroectoderm-specific genes fkd3, hoxa-1, and eng3, and the ectopic exp
ression of hoxa-1 was not inhibited by BMP4 overexpression. Kheper interact
ed with the transcriptional corepressors CtBP1 and CtBP2. Overexpression of
a Kheper mutant lacking the homeodomain or of a VP16-Kheper fusion protein
disturbed the development of the neuroectoderm and head structures. These
data underscore the role of Kheper in the development of the neuroectoderm
and indicate that Kheper acts as a transcriptional repressor. (C) 2000 Acad
emic Press.