A Colombian strain of Trypanosoma rangeli was characterized by analyzi
ng its behaviour in differ ent axenic and cellular culture, its infect
ion rate and the histopathological lesions produced in experimental an
imals. Although slight inflammatory infiltrations were shown in differ
ent histopathological sections, no pseudocysts could be observed Grace
's insect medium is better than liver infusion tryptose or artificial
triatomine urine supplemented with proline when studying T. rangeli me
tacyclogenesis, with a peak of 32% trypomastigotes. High infection rat
es were found in VERO and J774 cells. Because of its 100% infectivity
rates and adequacy of parasitemia levels, C23 strain is a suitable mod
el of T. rangeli biology study.