3 alpha-Hydroxy-5 alpha-pregnan-20-one exposure reduces GABA(A) receptor alpha 4 subunit mRNA levels

Authors
Grobin, AC Morrow, AL
Citation
Ac. Grobin et Al. Morrow, 3 alpha-Hydroxy-5 alpha-pregnan-20-one exposure reduces GABA(A) receptor alpha 4 subunit mRNA levels, EUR J PHARM, 409(2), 2000, pp. R1-R2
Citations number
8
Categorie Soggetti
Pharmacology & Toxicology
Journal title
EUROPEAN JOURNAL OF PHARMACOLOGY
ISSN journal
00142999 → ACNP
Volume
409
Issue
2
Year of publication
2000
Pages
R1 - R2
Database
ISI
SICI code
0014-2999(200012)409:2<R1:3AAERG>2.0.ZU;2-Y
Abstract
To examine the direct effects of neurosteroids on gamma -aminobutyric acid type A (GABA(A)) receptor expression, we exposed developing neuronal cells (P19) in vitro to 3 alpha -hydroxy-5 alpha -pregnan-20-one (3 alpha ,5 alph a -THP, allopregnanolone). Quantitative reverse transcriptase-polymerase ch ain reaction (RT-PCR) analysis revealed a concentration-dependent decrease in GABA(A) receptor alpha4 subunit mRNA expression that reversed 24 h after steroid withdrawal. These data suggest that variations in neurosteroid lev els regulate the pattern of GABA(A) receptor subunit expression and may alt er the trophic effects of GABA. (C) 2000 Elsevier Science B.V. All rights r eserved.