Screening of sulfate-reducing bacteria in colonoscopy samples from healthyand colitic human gut mucosa

A PCR-based approach combined with microbiological cultivation methods was employed to determine the occurrence of sulfate-reducing bacteria (SRB) in colon biopsy samples from ulcerative colitis patients and from non-colitic controls. The detection of mucosa-associated SRB was carried out by digoxig enin-dUTP-labelled PCR amplification, in liquid Postgate medium B and in a new liquid medium, termed VM medium I. Using Postgate medium B; the growth of SRB was confirmed in 92% of the colitic specimens and in 52% of non-coli tic samples. However, PCR analysis and incubation in VM medium I detected S RB in 100% of biopsy material indicating ubiquitous presence of SRB in huma n colon mucosa. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.