The effect of 15,30 and 60 atm CO2 pressure at 25,35 and 45 degrees C on Li
steria monocytogenes was studied. A biphasic curve was observed in the CO2
treatments. A primary curve was characterized with very little killing and
a secondary curve was showed straight-line inactivation kinetics. An increa
se of pressure and/or temperature enhanced the antimicrobial effects of CO2
. Listeria monocytogenes suspended in physiological saline containing 1% br
ain-heart infusion broth was completely inactivated under 60 atm CO2 treatm
ent in 115, 75 and 60 min at 25 35 and 45 degrees C, drespectively! A minim
um D-value was obtained under 60 atm CO;, pressure at 45 degrees C. High pr
essure CO2 treatment technique can possibly be applied to reduce microbiol
load in whole and skim milk;and carrot, orange, peach, and apple juices (C)
2000 Academic Press.