KR 31372, a benzopyran derivative, inhibits oxidized LDL-stimulated proliferation and migration of vascular smooth muscle cells

KR 31372 is a benzopyran derivative. Both [H-3]thymidine incorporation and migrations (chemotactic and wound-edge) of cultured smooth muscle cells (SM Cs) were greatly stimulated by oxidized low-density lipoprotein (LDL). Thes e effects were significantly suppressed by KR 31372 (10(-7)-10(-6) M) and P DGF-BB antibody (10(-8)-10(-6) M). Preincubation with KR 31372 led to a dec rease in the synthesis of PDGF-BB-like immunoreactivity (PDGF-BB-LI) that h ad been stimulated by oxidized LDL. Otherwise, KR 31372 and probucol strong ly inhibited the production of thiobarbituric acid reactive substances (TBA RS) caused by the incubation of LDL with Cu2+ ion, and significantly reduce d the intracellular oxidative stress when stimulated with H2O2. Taken toget her, it is suggested that KR 31372 may inhibit the oxidized LDL-stimulated syntheses of DNA and PDGF-BB, and migration of the SMCs, in part, via the a ntioxidant activity. (C) 2000 Editions scientifiques et medicales Elsevier SAS.