S. Martinek et Mw. Young, Specific genetic interference with behavioral rhythms in drosophila by expression of inverted repeats, GENETICS, 156(4), 2000, pp. 1717-1725
We describe a new experimental technique that allows for a tissue-specific
reduction of gene activity in the Drosophila. nervous system. On the basis
of the observation that certain gene functions can be ubiquitously blocked
by injecting double-stranded RNA into Drosophila embryos, we employed a met
hod to interfere with an individual gene function permanently in a predeter
mined cell type. This was achieved by the formation of an inverted-repeat R
NA sequence in the tissue of interest under control of the GAL4/UAS binary
expression system. As an example, we show that inverted-repeat-mediated int
erference with the period gene produces a hypomorphic period phenotype. A s
elective decrease of period RNA appears to be a component of the cellular r
esponse.