Mutator-like elements in Arabidopsis thaliana: Structure, diversity and evolution

While genome-wide surveys of abundance and diversity of mobile elements hav e been conducted for some class I transposable element families, little is known about the nature of class II transposable elements on this scale. In this report, we present the results from analysis of the sequence and struc tural diversity of Mutator-like elements (MULEs) in the genome of Arabidops is thaliana (Columbia). Sequence similarity searches and subsequent charact erization suggest that MULEs exhibit extreme structure, sequence, and size heterogeneity. Multiple alignments at the nucleotide and amino acid levels reveal conserved, potentially transposition-related sequence motifs. While many MULEs share common structural features to Mu elements in maize, some g roups lack characteristic long terminal inverted repeats. High sequence sim ilarity and phylogenetic analyses based on nucleotide sequence alignments i ndicate that many of these elements with diverse structural features may re main transpositionally competent and that multiple MULE lineages may have b een evolving independently over long time scales. Finally, there is evidenc e that MULEs are capable of the acquisition of host DNA segments, which may have implications for adaptive evolution, both at the element and host lev els.