Genomic organization of sheep TRDJ segments and their expression in the delta-chain repertoire in thymus

Authors
Massari, S Antonacci, R Lanave, C Ciccarese, S
Citation
S. Massari et al., Genomic organization of sheep TRDJ segments and their expression in the delta-chain repertoire in thymus, IMMUNOGENET, 52(1-2), 2000, pp. 1-8
Citations number
23
Categorie Soggetti
Immunology
Journal title
IMMUNOGENETICS
ISSN journal
00937711 → ACNP
Volume
52
Issue
1-2
Year of publication
2000
Pages
1 - 8
Database
ISI
SICI code
0093-7711(200011)52:1-2<1:GOOSTS>2.0.ZU;2-F
Abstract
cDNA sequences obtained from polymerase chain reaction products of reverse- transcribed RNA from sheep thymus showed the presence of a large number of members of the TRDV1 gene family. Some are TRDV1 genes also found in periph eral blood lymphocytes, while four genes had not been described so far. The cDNA sequences also showed extensive junctional diversity and a preferenti al usage of the three TRDJ elements. We characterized the genomic organizat ion of the sheep TRDJ locus and detected a correlation between the nonrando m usage of TRDJ elements during development and their chromosomal order.