CLONING AND IN-VITRO EXPRESSION OF RAT LECITHIN-CHOLESTEROL ACYLTRANSFERASE

Authors
WANG JC GEBRE AK ANDERSON RA PARKS JS
Citation
Jc. Wang et al., CLONING AND IN-VITRO EXPRESSION OF RAT LECITHIN-CHOLESTEROL ACYLTRANSFERASE, Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1346(3), 1997, pp. 207-211
Citations number
22
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta, L. Lipids and lipid metabolism → ACNP
ISSN journal
00052760
Volume
1346
Issue
3
Year of publication
1997
Pages
207 - 211
Database
ISI
SICI code
0005-2760(1997)1346:3<207:CAIEOR>2.0.ZU;2-E
Abstract
Rat lecithin:cholesterol acyltransferase (LCAT) cDNA was obtained by r everse transcriptase/polymerase chain reaction amplification of rat li ver total RNA. A consensus sequence was derived from four independent clones from two strains of rats. In vitro expression of rat LCAT cDNA in COS cells resulted in secreted enzyme protein with the same fatty a cyl specificity for phospholipase A(2) activity and cholesterol esteri fication as rat plasma LCAT, but different from that of recombinant or human plasma LCAT. (C) 1997 Published by Elsevier Science B.V.