Jc. Wang et al., CLONING AND IN-VITRO EXPRESSION OF RAT LECITHIN-CHOLESTEROL ACYLTRANSFERASE, Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1346(3), 1997, pp. 207-211
Rat lecithin:cholesterol acyltransferase (LCAT) cDNA was obtained by r
everse transcriptase/polymerase chain reaction amplification of rat li
ver total RNA. A consensus sequence was derived from four independent
clones from two strains of rats. In vitro expression of rat LCAT cDNA
in COS cells resulted in secreted enzyme protein with the same fatty a
cyl specificity for phospholipase A(2) activity and cholesterol esteri
fication as rat plasma LCAT, but different from that of recombinant or
human plasma LCAT. (C) 1997 Published by Elsevier Science B.V.