RELEASE OF AMINOPEPTIDASE FROM LACTOBACILLUS-CASEI SP CASEI BY CELL DISRUPTION IN A MICROFLUIDIZER

Authors
CHOI H LALEYE L AMANTEA GF SIMARD RE
Citation
H. Choi et al., RELEASE OF AMINOPEPTIDASE FROM LACTOBACILLUS-CASEI SP CASEI BY CELL DISRUPTION IN A MICROFLUIDIZER, Biotechnology techniques, 11(7), 1997, pp. 451-453
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
7
Year of publication
1997
Pages
451 - 453
Database
ISI
SICI code
0951-208X(1997)11:7<451:ROAFLS>2.0.ZU;2-Q
Abstract
Cell disruption in a Microfluidizer was a function of both operating p ressure and number or passes. The operating pressure bad greater effec t on the disruption than did the number or passes as indicated by the magnitude ol the constants from the cell disruption equation. Protein release correlated with aminopeptidase release by Lactobacillus casei sp. casei The optimum operating pressure for enzyme extraction was 76 MPa with loss of enzyme activity about 15 to 20%.