DIFFERENT METHANOL FEEDING STRATEGIES TO RECOMBINANT PICHIA-PASTORIS CULTURES PRODUCING HIGH-LEVEL OF DEXTRANASE

Authors
JIMENEZ ER SANCHEZ K ROCA H DELGADO JM
Citation
Er. Jimenez et al., DIFFERENT METHANOL FEEDING STRATEGIES TO RECOMBINANT PICHIA-PASTORIS CULTURES PRODUCING HIGH-LEVEL OF DEXTRANASE, Biotechnology techniques, 11(7), 1997, pp. 461-466
Citations number
17
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
7
Year of publication
1997
Pages
461 - 466
Database
ISI
SICI code
0951-208X(1997)11:7<461:DMFSTR>2.0.ZU;2-V
Abstract
Three different strategies to control the methanol flow rate in cultur es of a dextranase-producing Mut(s) clone of the methylotrophic yeast Pichia pastoris were analysed. The cell growth and secretion of dextra nase were different for each strategy. The increment of methanol-feedi ng rate used in the strategy based on dissolved oxygen (DO) in the cul ture medium gave better results for both cell growth and enzyme secret ion, reaching 56.2 g dry biomass and 5.14 g of dextranase per litre of broth.