USE OF FLOW-CYTOMETRY TO DETECT GENOTOXINS BY THE SALMONELLA SULA-TEST

Authors
ELMZIBRI M GUIRAUDDAURIAC H LAGET M BEUDOT C DEMEO M DUMENIL G
Citation
M. Elmzibri et al., USE OF FLOW-CYTOMETRY TO DETECT GENOTOXINS BY THE SALMONELLA SULA-TEST, Biotechnology techniques, 11(7), 1997, pp. 467-470
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
7
Year of publication
1997
Pages
467 - 470
Database
ISI
SICI code
0951-208X(1997)11:7<467:UOFTDG>2.0.ZU;2-B
Abstract
The Salmonella sulA-test using Salmonella typhimurium TA1538/pEM1968 ( sulA::lacZ) is a new SOS-repair inducing system that detects mutagens and carcinogens. The beta-galactosidase activity, currently detected b y colorimetric dosage, can be measured by flow cytometry using a fluor escent substrate (fluorescein-di-beta-D-galactopyranoside). Comparison of the dose-response relationships of eight chemicals determined by t he two techniques showed that the Salmonella sulA-test combined with t he flow cytometry technique was accurate and reliable as it covered a large number of cells in a short time.