DNA DELIVERY INTO RICE CELLS AND TRANSFORMATION USING SILICON-CARBIDEWHISKERS

Authors
NAGATANI N HONDA H SHIMADA T KOBAYASHI T
Citation
N. Nagatani et al., DNA DELIVERY INTO RICE CELLS AND TRANSFORMATION USING SILICON-CARBIDEWHISKERS, Biotechnology techniques, 11(7), 1997, pp. 471-473
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
7
Year of publication
1997
Pages
471 - 473
Database
ISI
SICI code
0951-208X(1997)11:7<471:DDIRCA>2.0.ZU;2-C
Abstract
A plasmid (pAct1-F), containing beta-glucuronidase (GUS) as a reporter gene, was delivered into embryogenic rice cells by using silicon carb ide whiskers (SCW). The cells were thoroughly mixed with the plasmid a nd SCW, incubated for 1 day and transient GUS activity was revealed hi stochemically. Under optimal conditions, 533 transformants per 1 g wet cells were observed.